Detection and distribution of rotavirus in raw sewage and creeks in Sao Paulo, Brazil
Article Abstract:
The distribution of rotaviruses were detected and quantified in domestic sewage and sewage-polluted creeks in the city of Sao Paulo, Brazil. Eight litersamples were absorbed and eluted from positively charged microporous filters and ultracentrifugated of their filter eluates. Rotavirus were detected and enumerated by indirect immunofluorescence and direct immunoperoxidase methods. The seasonal variation of the virus corresponded to the seasonal variation of diarrhea in the city.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Electropositive filter membrane as an alternative for the elimination of PCR inhibitors from sewage and water samples
Article Abstract:
Electropositive membrane filtration can eliminate many organic and inorganic compounds in environmental samples that can interfere with PCR analysis. Researchers used both fluorpentane treatment and a ZP60S electropositive filter membrane to isolate enteric viruses from sewage and sewage-polluted water.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
TaqMan PCR for detection of Vibrio cholerae O1, O139, non-O1, and non-O139 in pure cultures, raw oysters, and synthetic seawater
Article Abstract:
Researchers describe a TaqMan PCR probe that can distinguish all strains of Vibrio cholerae and exclude all other Vibrio species in oysters and seawater. The probe is based on the nonclassical hemolysin hlyA sequence and can detect viable but nonculturable strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Inheritance and distribution of trichome phenotypes in Datura wrightii. Base composition of Coffea AFLP sequences and their conservation within the genus
- Abstracts: A double-selective tissue culture system for isolation of wild-type poliovirus from sewage applied in a long-term environmental surveillance
- Abstracts: Isolation and purification of propionicin PLG-1, a bacteriocin produced by astrain of Propionibacterium thoenii
- Abstracts: RNA-protein interactions in regulation of picornavirus RNA translation. Suppression of cap-dependent translation in mitosis
- Abstracts: Isolation of Pseudomonas picketti strains that degrade 2,4,6-trichlorophenoland their dechlorination of chlorophenols