Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria

Article Abstract:

Research analyzes the distribution of human intestinal bifidobacteria in intestinal tracts by a combination of real-time polymerase chain reaction (PCR) with Bifidobacterium genus- and species-specific primers. Results show that the procedure can also be applied to detect Bifidobacterium in feces. Three groups, Bifidobacterium adolescentis group, B. catenulatum group, and B. longum are idetified.

Science & research, Goods & services distribution, Channels of Distribution, Usage, Distribution, Identification and classification, Statistics, Statistics (Data), Company distribution practices, Bifidobacteria

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Species-specific oligonucleotide probes for five Bifidobacterium species detected in human intestinal microflora

Article Abstract:

Species-specific oligonucleotide probes were designed from synthesized DNA probes closely related to five species of the genus Bifidobacterium. Extracted bacterial RNAs and fixed whole cells were used to test the specifity of the probes. Results ascertained the value of the probes in rapid clinical diagnostic tests for the identification and detection of human intestinal Bifidobacterium species.

Analysis, Nucleic acid probes

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Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers

Article Abstract:

Research was conducted to examine the distribution of Bifidobacteria in the human intestine using a species-specific molecular probe and polymerase chain reaction technique. Results show the preponderance of Bifidobacterium catenulatum over other species in the human fecal samples.

Methods, Physiological aspects, Observations, Feces, Bacterial genetics, Host-bacteria relationships, Gastrointestinal system

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