Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases
Article Abstract:
Thermosensitive mutant strains of Saccharomyces cerevisiae were molecularly analyzed to study the mitotic double-strand break (DSB)-induced gene conversion at mating-type in the yeast. Results showed that the DSB repair is greatly inhibited by mutants lagging strand replication, namely, DNA polymerase alpha, DNA primase and Rad27p. Based on the results, a novel model was developed for DSB-induced gene conversion in which a strand invasion yields a modified replication that is terminated by the arrest of the second end of the DSB.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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A novel mutation avoidance mechanism dependent on S. cerevisiae RAD27 is distinct from DNA mismatch repair
Article Abstract:
Mutations in the Saccharomyces cerevisiae RAD27 gene produce a strong mutator phenotype. Most of these resulting mutations were found to have a structure in which sequences ranging from 5-108 bp flanked by direct repeats of 3-12 bp were duplicated. Findings indicated that most of replication errors that accumulate in rad27 strains are processed by double-strand break repair, while a smaller portion are processed by a mutagenic repair pathway.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Saccharomyces Ku70, Mre11/Rad50, and RPA proteins regulate adaptation to G2/M arrest after DNA damage
Article Abstract:
An unrepairable double-strand break in the chromosome of the yeast Saccharomyces cerevisiae leads to an arrest in the cell cycle just between the second growth phase and the cell division phase. The arrest at this stage of the cell cycle is regulated by a number of proteins that either protect DNA from degradation or at least retard enzymatic digestion of the broken fragments of DNA.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
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