Expression of the flagellin gene in Borrelia is controlled by an alternative sigma factor
Article Abstract:
Cloning, sequencing and molecular analysis of the flagellin genes from two relapsing fever species Borrelia hermsii and Borrelia crocidurae, the avian borreliosis agent Borrelia anserina and three Lyme disease genomic species, Borrelia burgdorferi, Borrelia garinii and Borrelia afzelii, reveal a sequence identity of 85 to 93% amongst all the species. Promoter regions, ribosome binding sites and transcription initiation points of these genes are determined from nucleotide sequencing and primer extension on isolated mRNA from B. hermsii and B. burgdorferi. The Borrelia fla gene promoter region resembles the bacteriophage SP01 sigma(gp33-34) promoter sequence of Bacillus subtilis.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Transcriptional and translational regulation of the expression of the major outer surface proteins in Lyme disease Borrelia strains
Article Abstract:
The nucleotide sequence of the operon in Borrelia afzelii strain F1 is almost identical to that in ACAI strain but the expression of the surface protein in the strains F1, ACAI and B31 differ considerably. Strain F1 unlike the strains ACAI and B31 produces the outer surface protein OspC and does not produce OspA and OspB proteins. The ospA and ospB genes are present on one operon and gene expression is regulated at the transcriptional and translational levels since in F1 strain the amount of ospAB mRNA varies more than the amount of protein and there is a inverse relation between ospAB and ospC operons.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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The purB gene of Escherichia coli K-12 is located in an operon
Article Abstract:
The purB gene of Escherichia coli chromosome was cloned and sequenced to determine its transcription regulators. The purB gene for succinyl-adenosine monophosphate (A-AMP) lyase is the second gene of the two-gene transcription-linked promoter which is composed of a pur operator and a DnaA box. The gene exhibited regulatory functions and it is co-transcribed with the ycfC upstream gene for encoding a membrane-associated protein.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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