Fur is involved in manganese-dependent regulation of mntA (sitA) expression in Sinorhizobium meliloti

Article Abstract:

The fur is a transcriptional supervisor, which is a part of the iron based control of the appearance of gene in a lot of bacterias. The information shows a new role for the bacterial fur protein in reconciling the directive for the appearance of gene that is dependent on manganese.

Primary nonferrous metals, not elsewhere classified, Primary Smelting and Refining of Nonferrous Metal (except Copper and Aluminum), Manganese, Fur, Sweet clover, Fur (Anatomy)

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Sinorhizobium melitoli Fur-like (Mur) protein binds a Fur box-like sequence present in the mntA promoter in a manganese-responsive manner

Article Abstract:

The [Mur.sub.Sm] protein binding o the promoter region of the Sinorhizobium melitoli mntA gene was analyzed. Results indicate that two monomers or one dimer, of [Mur.sub.Sm] binds the DNA, however manganese activated [Mur.sub.Sm] binds with low affinity to a canonical Fur box.

Physiological aspects, Genetic aspects, Mitochondrial DNA, DNA-ligand interactions, DNA binding, Rhizobium meliloti

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Modular broad-host-range expression vectors for single-protein and protein complex purification

Article Abstract:

A set of modular broad-host-range expression vectors was created and vectors were mobilized by conjugation. These tools are useful for protein purification and for studying protein-protein interactions in a range of bacterial species.

Methods, Protein binding, Protein preparations

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