Growth rate regulation of rRNA content of a marine Synechococcus (cyanobacterium) strain
Article Abstract:
Research was conducted to analyze the cellular rRNA content of a coastal Synechococcus isolate growing at a range of light-limited growth rates Fluorescently labeled 16S rRNA-targeted oligonucleotide probes were used in the analysis while nucleic acid stain SYBR Green II was used for the measurement of total RNA. Flow cytometry was also used for a rapid analysis of stained and hybridized cells. Results indicate that calculations of cellular rRNA content can prove useful for the measurement of in situ growth rates in natural Synechococcus populations in mixed microbial communities.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus
Article Abstract:
A plating method is proposed for obtaining isolated colonies of Synechococcus sp. strains WH7803, WH8102 and WH8103 at high efficiencies. The method uses a very low concentration of washed agar and allows discrimination between motile and nonmotile colonies. It is complemented by a technique for introducing a replicative vector and a suicide vector in the Synechococcus strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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