The binding pattern of two carbohydrate-binding modules of laminarinase Lam16A from Thermotoga neapolitana: differences in beta-glucan binding within family CBM4

Article Abstract:

Research shows that family of carbohydrate-binding modules, comprising complex hydrolytic extracellular bacterial enzymes, possess different polysaccharide-binding specificities. Two modules flank Laminarinase Lam16A from Thermotoga nepolitana, which bind to different glucan molecules such as soluble laminarin and barley glucan at the N-terminus and curdlan, pustulan and yeast glucan at the C-terminus.

Statistical Data Included, Microbiological synthesis, Microbial polysaccharides, Protein binding, Bacterial cell walls

---

Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-beta-1,3-glucanase bound to the outer cell surface

Article Abstract:

Research has been conducted on Clostridium thermocellum which produced beta-1,3-glucanase. The authors report that Lic16A protein has been located on the cell surface, and that its production allowed C. thermocellum to grow on beta-1,3- or beta 1,3-1,4-glucan.

Science & research, Research, Genetic aspects, Cells (Biology), Growth, Clostridium, Microbiology, Cells, Genetic research, Company growth

---

Highly thermostable endo-1,3-beta-glucanase (laminarase) LamA from Thermotoga neapolitana: nucleotide sequence of the gene and characterization of the recombinant gene product

Article Abstract:

The primary structure of the gene and the LamA protein of Thermotoga neapolitana was analyzed to determine the relationship between 1,3-beta- and 1,3-1,4-beta-glucanases. Analysis of the purified recombinant LamA protein fractions from Thermotoga neapolitana indicated the presence of enzymatic and purification profiles that were similar to the 52 kilodalton (kDa) glucanase. The purified 52 kDa beta-glucanase also resembled the processed form of the 73 kDa recombinant LamA which exhibited high specificity towards laminarin.

Nucleotide sequence, Base sequence, Microbial enzymes

Similar abstracts:

• Abstracts: A novel thermostable multidomain 1,4-beta-xylanase from 'Candibacillus cellulovorans' and effect of its xylan-binding domain on enzyme activity
• Abstracts: Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp
• Abstracts: Thermostable NADP(super +)-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp. strain M-1: purification and characterization and gene expression in Escherichia coli
• Abstracts: Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar
• Abstracts: SWI/SNF-dependent chromatin remodeling of RNR3 requires TAF (sub)II s and the general transcription machinery

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.