Identification distinct communities of sulfate-reducing bacteria in oil fields by reverse sample genome probing
Article Abstract:
The reverse sample genome probing (RSGP) procedure was used to assess the population profile of sulfate-reducing bacteria (SRB) in oilfields of Alberta, Canada. The procedure employed 35 different standards of sulfate-reducing bacteria, which were used to characterize samples from 56 sites. Of the 35 standards, 34 were detected, and statistical analysis indicated the presence oftwo distinct communities of SRB in 44 of the 56 sites. These two communities correlated well with the salt concentration of water, which were either freshwater or saline.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Deletion of the rbo gene increases the oxygen sensitivity of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough
Article Abstract:
Research was conducted to examine the hypothesis that the deletion of the rbo gene increases the oxygen sensitivity of the sulfate-reducing bacterium Desulfovibrio vulgaris hildenborough. The rbo gene was deleted from D. vulgaris with a previously developed sacB mutagenesis procedure. Results indicate that Rbo serves as an oxygen defense protein in the anaerobic, sulfate-reducing bacterium D. vulgaris Hildenborough and likely also in other sulfate-reducing bacteria and anaerobic archaea in which it has been found.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Identification of hydrocarbon-degrading bacteria in soil by reverse sample genome probing
Article Abstract:
The study is conducted to determine the effectiveness of reverse sample genome probing (RSGP) as a tool for assessing the impact of the introduction of unsaturated hydrocarbons on a soil microbial community. The RSGP procedure is used to analyze the effects of the highly degradable compound toluene and the highly recalcitrant compound dicyclopentadiene on community composition. The technique is found to be effective in identifying bacteria which metabolize degradable or recalcitrant hydrocarbons.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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