Identification of pyruvate carboxylase genes in Pseudomonas aeruginosa PAO1 and development of a P. aeruginosa-based overexpression system for [alpha.sub.4]- and [alpha.sub.4][beta.sub.4] -type pyruvate carboxylases
Article Abstract:
An [alpha.sub.4]- type and an [alpha.sub.4][beta.sub.4] -type pyruvate carboxylase (PYC), for which Escherichia coli is not a suitable host, that can be overexpressed recombinantly in Pseudomonas aeruginosa, is presented. Pseudomonas aeruginosa type strain that allowed the expression of recombinant PYCs in the absence of the native enzyme, is developed which can be transformed genetically and lysed for cell extract preparation easily.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Autotransporter-encoding sequences are phylogenetically distributed among Escherichia coli clinical isolates and references strains
Article Abstract:
The development and validation of a multiplex PCR assay to screen autotransporter genes in Escherichia coli showed that the autotransporter gene profiles might be useful for comparative analysis of Escherichia coli strains from clinical, food, and environmental sources.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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