Effect of inoculation and leaf litter amendment on establishment of nodule-forming Frankia populations in soil
Article Abstract:
Researchers show that soil can be inoculated with strains of the actinomycete Frankia that are high nitrogen fixers in the absence of the host plant and the strain will maintain its nitrogen-fixing ability. These strains can successfully compete with the indigenous Frankia species and form nodules that increase plant growth.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Detection of mRNA in Streptomyces cells by whole-cell hybridization with digoxigenin-labeled probes
Article Abstract:
Whole-cell hybridization with digoxigenin-labeled in vitro transcripts followed by an antibody-alkaline phosphatase detection of the digoxigenin reporter molecular helped trace the mRNA of the thiostrepton resistance gene (tsr) harbored by plasmid PIJ673 in streptomyces violacelatus. Before hybridization, the cells had to be permeabilized by lysozyme, the detergent Nonidet P-40 and toulene. Hybridization with digoxigenin-labeled, 16S rRNA-targeted oligonucleotides indicated the permeability of the Streptomyces violacelatus cells for probes and the antibody-alkaline phosphatase conjugate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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In situ analysis of denitrifying toluene- and m-xylene-degrading bacteria in a diesel fuel-contaminated laboratory aquifer column
Article Abstract:
An aquifer contaminated with diesel fuel was bioremediated in situ by the introduction of oxidants such as O2 and NO3- and nutrients to stimulate microbial activity. An aquifer sample was extracted after 3.5 years of remediation to determine bacterial strains which can flourish on selected hydrocarbons under denitrifying conditions, create a laboratory aquifer column to induce the aerobic and denitrifying processes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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- Abstracts: Identification and expression of benzylsuccinate synthase genes in a toluene-degrading methanogenic consortium
- Abstracts: In situ hybridization of Prochlorococcus and Synechococcus (marine cyanobacteria) spp. with rRNA-targeted peptide nucleic acid probes
- Abstracts: Genotypic analyses of Escherichia coli O157:H7 and O157 nonmotile isolates recovered from beef cattle and carcasses at processing plants in the Midwestern states of the United States
- Abstracts: Impact of seasonal variations and nutrient inputs on nitrogen cycling and degradation of hexadecane by replicated river biofilms
- Abstracts: Induction of bphA, encoding biphenyl dioxygenase, in two polychlorinated biphenyl-degrading bacteria, psychrotolerant Pseudomonas strain Cam-1 and mesophilic Burkholderia strain LB400