Translational control of puf operon expression in Rhodobacter sphaeroides 2.4.1

Article Abstract:

The puf operon of Rhodobacter sphaeroides 2.4.1 can be translated. A series of pufK-specific mutations proved that translation initiation through pufK may play an important role in the translation initiation at pufB, either through the frequency of occurrence of rare codons in pufK, their absolute numbers, their absolute identity or any combination of these factors. Data also indicate that ribosome movement through pufK can eventually impact on the final intracellular level of the B875 spectral complex by restricting the level of B875 beta-polypeptide.

Gene expression, Operons

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Identification and molecular genetic analysis of multiple loci contributing to high-level tellurite resistance in Rhodobacter sphaeroides 2.4.1

Article Abstract:

A research was conducted to study the cloning, sequence and inactivation of tellurite resistance determinants from Rhodobacter sphaeroides 2.4.1. Standard protocols were utilized for DNA isolation and recombinant DNA procedures while plasmids were characterized in biparental matings from Escherichia coli S17-1 strains into R. sphaeroides strains. Results showed that the disruption of the R. sphaeroides 2.4.1 cysK gene impaired tellurite resistance levels and suggested that there may be a link between cysteine biosynthesis and tellurite resistance.

Metabolic regulation, Molecular genetics, Tellurium, Cysteine

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Isolation of regulatory mutants in photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1 and partial complementation of a PrrB mutant by the HupT histidine-kinase

Article Abstract:

The photosynthetic bacteria Rhodobacter spheroides increases the expression of photosynthesis genes during the transition from aerobiosis to anaerobic photosynthesis. Complementation analysis and DNA sequencing of the mutation in D5, a mutant of the photosynthetic bacterium Rhodobacter (R.) sphaeroides, indicates that the mutation is present in the photosynthetic response regulator sensor kinase, (prrB), that can regulate the expression of the photosynthetic gene.

Genetic aspects, Mutation (Biology), Mutation, Bacteria, Photosynthetic, Photosynthetic bacteria

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