In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants
Article Abstract:
The modifications of earlier published chromatin immunoprecipitation assay, is used to show that cotranscriptional splicing occurs approximately 1 kb after transcription of 3' splice site. This protects intron-containing nascent transcripts from effects of cleavage by intronic hammerhead ribozyme and suggests that high percentage of introns are recognized cotranscriptionally.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2006
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Insights into the mechanism of splicing: More lessons from the ribosome
Article Abstract:
Splicing catalysts proceeds through two consecutive transesterifications involving three sites of the intron. A detailed understanding of the mechanism is necessary for ultimate explanation of all aspects of the splicing from its evolutionary origins to the regulation of alternative splicing.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2005
User Contributions:
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