Insertional gene inactivation in a phototrophic sulphur bacterium: APS-reductase-deficient mutants of Chromatium vinosum

Article Abstract:

The cloning of the genes encoding the adenylylsulphate-forming enzyme adenosine-5'-phosphosulphate (APS) reductase from Chromatium vinosum strain D (DSM 180), a representative of the purple sulphur bacteria, and the construction of mutations in these genes via insertion of a kanamycin omega cartridge are described. The first insertional gene inactivation in purple sulphur bacteria was verified through southern hybridization and PCR analyses of the recombinants obtained. The absence of APS reductase from the mutants was noted, but this was found to have no significant effect on the sulphite-oxidizing ability of the cells under photolithoautotrophic growth conditions.

Physiological aspects, Cloning, Sulfur bacteria

---

Truncation as a novel form of variation of the p50 gene in Mycoplasma hominis

Article Abstract:

Research was conducted to examine the existence of a variant of the p50, an adhesin molecule, in Mycoplasma hominis. To investigate the ubiquity of P50 in M hominis and to determine if the isolates, which did not react with the mAb, contain and express the gene encoding P50, the isolates were studied at the mRNA and DNA level. Results indicated that p50 gene sequences were present in all M hominis isolates that were examined.

Genetic aspects, Antigens, Mycoplasmatales

---

Construction of a single-copy integration vector and its use to study gene expression in bacillus licheniformis

Article Abstract:

Bacillus licheniformis is used to produce peptide antibiotic bacitracin (Bt) alpha-amylase, beta-lactamase, alkaline protease and alkaline phosphatase. A versatile system to study gene expression in single copy is reported, integrated via a Campbell type system into the heterologous bts locus, comprising a promote-probe pTLH vector and Lac- mutant derivative of B. licheniformis.

Bacitracin

Similar abstracts:

• Abstracts: Quantification of microscopic feather characters used in the identification of North American plovers
• Abstracts: Stress-induced transcriptional activation. Double-stranded RNA viruses of Saccharomyces cerevisiae. High-osmolarity signalling in Saccharomyces cerevisiae is modulated in carbon-source-dependent fashion
• Abstracts: Variation in resistance to hydrostatic pressure among strains of food-borne pathogens. Construction of a range of derivatives of the biological control strain Agrobacterium rhizogenes K84: a study of factors involved in biological control of crown gall disease
• Abstracts: Aspartate carbamoyltransferase from a psychrophilic deep-sea bacterium, Vibrio strain 2693: properties of the enzyme, genetic organization and synthesis in Escherichia coli
• Abstracts: Molecular characterization of a toluene-degrading methanogenic consortium. The methanogenic archaeon Methanosarcina thermophila TM-1 possesses a high-affinity glycine betaine transporter involved in osmotic adaptation

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.