Isolation, characterization, and heterologous expression of the novel lantibiotic epicidin 280 and analysis of its biosynthetic gene cluster
Article Abstract:
Epicidin 280, a lantibiotic produced by Staphylococcus epidermidis and related to the lantibiotic Pep5, was isolated and characterized and its biosynthetic gene cluster was analyzed. A putative oxidoreductase gene was identified in the gene cluster and was found to be capable of reducing an N-terminal oxopropionyl residue in epicidin 280. Results demonstrate that epicidin 280 generated by the heterologous host Staphylococcus carnosus behaved homogeneously during reverse-phase chromatography.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Molecular analysis of expression of the lantibiotic Pep5 immunity phenotype
Article Abstract:
Research was conducted to examine the molecular characterization of the Pep5 immunity system. The immunity gene pepI was localized upstream of the structural gene pepA. The tight coupling of the immunity phenotype and Pep5 production was observed. Results based on Western blot analysis suggest that F13D PepI and PepI 1-65 were not produced correctly or were partially degraded while I17R PepI was found to be less efficient in offering self-protection than the wild-type PepI.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Localization and functional analysis of PepI, the immunity peptide of Pep5-producing staphylococcus epidermidis strain 5
Article Abstract:
Pep5 is a lantibiotic, a subgroup of bacteriocins from gram-positive bacteria, is produced by staphyococcus epidermidis strain 5. In the experiment conducted the immunity peptide of Pep5 is examined and analysis of PepI is made.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Identification, characterization, and in situ detection of a fruit-body-specific hydrophobin of Pleurotus ostreatus
- Abstracts: Identification and characterization of a tandem repeat in exon III of the dopamine receptor D4 (DRD4) gene in cetaceans
- Abstracts: Impact of heterologus expression of Escherichia coli UDP-glucose pyrophosphorylase on trehalose and glycogen synthesis in Corynebacterium glutamicum
- Abstracts: Isolation and characterization of a cyanophage infecting the toxic cyanobacterium Microcystis aeruginosa. Isolation and characterization of a novel single-stranded RNA virus infecting the bloom-forming diatom rhizosolenia setigera
- Abstracts: Dynamics and biodiversity of populations of lactic acid bacteria and acetic acid bacteria involved in spontaneous heap fermentation of cocoa beans in Ghana