Isolation of lightning-competent soil bacteria
Article Abstract:
The isolation of two "lightning competent" soil bacteria after direct electroporation of the Nycodenz bacterial ring extracted from prairie soil in the presence of the pBHCRec plasmid (Tcr, Spr, Smr) is reported. Study demonstrated that the bacterial community directly isolated from soil by Nycodenz extraction constituted an effective pool of bacteria for finding examples of those that were lightning competent.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Strategy for in situ detection of natural transformation-based horizontal gene transfer events
Article Abstract:
A strategy is described that has enabled the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Fluorescence microscopy is used for the microscale detection of bacterial transformants growing on plant tissues and cultivation-based selection of transformants on antibiotic-containing agar plates has underestimated transformation frequencies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2008
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Natural electrotransformation of lightning-component Pseudomonas sp. strain N3 in artificial soil microcosms
Article Abstract:
The lightning-component Pseudomonas sp. strain N3, isolated from soil, is used to study the extent of natural electrotransformation (NET) or lightening transformation as a horizontal gene transfer mechanism in soil. Based on the range of the electric field that induces NET of Pseudomonas strain N3, the volume of soil, where NET could occur, ranges from 2 to 950 [m.sup.3] per lightening strike.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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