Manipulation of independent synthesis and degradation of polyphosphate in Escherichia coli for investigation of phosphate secretion from the cell
Article Abstract:
Plasmids that facilitate the controlled expression of ppk and ppx genes were constructed and expressed in an Escherichia coli strain to determine the genetic mechanisms of polyphosphate synthesis and degradation. Polyphosphate extraction, enzyme assays and RNA analysis indicated the ability of ppk- and ppx-expressing Escherichia coli strain to produce active polyphosphatekinase (PPK) after the induction of the ppx gene. Furthermore, the growth of phosphate-starved cells was promoted by the phosphate that was released from PPK due to the down-regulation of the phosphate-starvation response.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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The quiescent-cell expression system for protein synthesis in Escherichia coli
Article Abstract:
Protein synthesis in a metabolically active cell culture may be carried out in the absence of rapid cell division through the use of the quiescent-cell expression system. By evaluating the protein sequence of the Escherichia coli strain, it was found that quiescent cells are created by the controlled overexpression of Rcd in hns-205 mutant cells in broth culture. A significant decrease in the chromosomal gene expression of the strain was also cited, although its capacity to maintain protein synthesis remains active.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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In vivo labeling of Eschirichia coli cell envelope proteins with N-hydroxysuccinimide esters of biotin
Article Abstract:
The selective labeling capabilities of succinimidyl-6-biotinamido-hexanoate and sulfosuccinimidyl-6-biotinamido-hexanoate were tested. The membrane, periplasmic and cystolic fractions of Escherichia coli strains were subjected to probe localization. Protein biotynlation was compared to protein profiles from previous studies. Results show that increased osmolarity affects changes in the outer membrane structure.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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