Mannitol-1-phosphate dehydrogenase from Cryptococcus neoformans is a zinc-containing long-chain alcohol-polyol dehydrogenase

Article Abstract:

Mannitol-1-phosphate dehydrogenase (MPD) from Cryptococcus neoformans has been found to be a zinc-containing long-chain alcohol-polyol dehydrogenase. The hypothesis that mannitol synthesis is required for wild-type stress tolerance and virulence in C. neoformans has not been tested directly. MPD has been purified and characterized from C. neoformans and the corresponding cDNA and gene, MPD1, cloned and sequenced. With the MPD1 available, testing of two hypotheses would be possible. One is that MPD is needed for mannitol biosynthesis, and the other is that the ability to synthesize mannitol is required for wild-type stress tolerance and virulence.

Statistical Data Included, Dehydrogenases, Oxidoreductases, Cytochemistry, Yeast, Yeast (Food product)

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Overexpression of a dominant-negative allele of YPT1 inhibits growth and aspartyl protease secretion in Candida albicans

Article Abstract:

Overexpression of a dominant-negative allele of YPT1 has been found to inhibit Candida albicans growth and aspartyl protease secretion. The long-term goal of the study, in which the C. albicans homologue of S. cerevisiae YPT1 was cloned, was to characterize key steps in the processing and secretion of C. albicans virulence-associated proteins. Molecular genetic approaches were used to investigate the gene and its function and to look at its effects on Sap secretion.

Genetic aspects, Proteins, Microbial enzymes, Opportunistic infections, Candida albicans

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Stress tolerance and pathogenic potential of a mannitol mutant of Cryptococcus neoformans

Article Abstract:

A Cryptococcus neoformans mutant (Cn MLP) that generates low quantities of mannitol was developed by UV irradiation from C. neoformans H99 (Cn H99). Cn MLP was more vulnerable than Cn H99 to growth inhibition and killing by heat and high sodium chloride concentrations. Findings indicated that the capability of C. neoformans to generate and accumulate mannitol may affect its tolerance to heat and osmotic stresses and its pathogenicity to mice.

Pathogenic microorganisms

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