Microsatellite markers in white-tailed deer

Article Abstract:

A genomic library is constructed with genomic DNA taken from the white blood cells of four white-tailed deer. Fourteen microsatellite clones were separated to be genetic markers for forensic applications. Individual fragments of DNA obtained from the deer are enlarged in a Techne MW-2 microliter format thermal cycler or an Ericomp Twin Block thermal cycler. The study shows that two clones provide long repeats and, when the PCR products are electrophoresed on acrylamide gels, show complex banding patterns. Five polymorphic markers were tested against other related species' DNA to determine unique karyotypes.

White-tailed deer

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Localization of the U2 linkage group of horses to ECA 3 using chromosome painting

Article Abstract:

Chromosome painting using probes for human chromosomes 4 and 16, HSA 4 and HSA 16, respectively, was performed to localize the U2 linkage group in the horse chromosome which has been recognized to contain genes that are also found in the aforementioned human chromosomes. The results demonstrated that only horse chromosome 3, designated ECA 3, hybridized with the HSA 4 and HSA 16 probes, indicating that the equine U2 linkage group is localized in ECA 3, spanning the centromere.

Usage, Horses, Chromosome mapping

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Linkage analysis of five new genetic markers of the Oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae)

Article Abstract:

Linkage analysis of the oriental fruit fly, Bactrocera dorsalis showed three genetic markers associated with linkage groups. The mandarin red gene was associated with the matte gene while the white eye and amethyst loci were linked to the white purparium gene. The yellow eye was found to be related with the third linkage group. These findings are significant in the development of autocidal techniques for an effective sterile insect release method.

Fruit-flies

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