Molecular and immunological characterization of OprL, the 18 kDa outer-membrane peptidoglycan-associated lipoprotein (PAL) of Pseudomonas aeruginosa
Article Abstract:
The oprL gene encoding the Pseudomonas aeruginosa peptidoglycan-associated lipoprotein (PAL) was cloned and analyzed to determine the molecular and immunological properties of the 18 kiloDalton (kDa) outer membrane protein. Western blot analysis of the Pseudomonas aeruginosa cosmid library indicated the presence of a 504 bp open reading frame (ORF) that encoded a protein for lipoprotein signal recognition. The ORF upstream to the oprL gene also encoded a 52 C-terminal amino acid that exhibited a high degree of homology to the TolB protein of Escherichia coli.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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The outer membrane of lipid A-deficient Escherichia coli mutant LH530 has reduced levels of OmpF and leaks periplasmic enzymes
Article Abstract:
The thermosensitive and lipid A-deficient Escherichia coli LH530 mutant was analyzed to characterized the mechanisms that mediate changes in outer membrane protein profiles and phospholipid biosynthesis. The lipid A-deficient Escherichia coli LH530 mutant was highly sensitive to hydrophobic antibiotics and produced reduced amounts of lipopolysaccharides. The outer membrane of the mutant was deficient in OmpF porins at higher temperatures. Furthermore, the thermosensitive LH530 leaked periplasmic beta lactamase.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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