Monitoring a widespread bacterial group: in situ detection of planctomycetes with 16S rRNA-targeted probes

Article Abstract:

The analysis of the natural profusion observed in planctomycetes by in situ hybridization was undertaken using two developed ribosomal RNA-targeted (rRNA) oligonucleotide probes. Planctomycetes are aerobic, Gram-negative chemoheterotrophic bacteria having ovoid, pear-shaped or spherical cell morphologies. The probe design was based on 16S rRNA sequences present in the rRNA database of the Technical University Munich in Germany. The probes developed were termed PLA46 and PLA886. The combined use of the two probes provided an accurate in situ identification of the organism's abundance.

Methods, Usage, Gram-negative bacteria, In situ hybridization, Nucleic acid probes

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Population analysis in a denitrifying sand filter: conventional and in situ identification of Paracoccus spp. in methanol-fed biofilms

Article Abstract:

An analysis of the microbial community of a denitrifying sand filter in a waste water treatment plant revealed that members of the genera Paracoccus and Hyphomicrobium are actively involved in methanol utilization and denitrification. Members of the genus Paracoccus are predominant among the isolates. Almost 50% of all denitrifying isolates hybridized with genus-specific probes of Paracoccus. However, the isolates failed to hybridize with species-specific probes of Paracoccus, indicating the presence of a new species within the genus Paracoccus.

Analysis, Bacteria, Sewage, Identification and classification, Denitrification

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Structure and function of a nitrifying biofilm as determined by in situ hybridization and the use of microelectrodes

Article Abstract:

Nitrosomonas, ammonia oxidizers, form a thick layer of cell clusters at the top of a nitrifying biofilm in a trickling filter of an aquaculture water recirculation system. Nitrobacter, nitrite oxidizers, form a less-dense layer close to the clusters of ammonia oxidizers on the biofilm. Nitrification is observed only in a narrow zone of 50 micrometer in the upper portion of the biofilm. Both the species are predominant in the oxic zone of the biofilm, but are also present in lesser quantity in anoxic zones.

Observations, Bacteria, Nitrifying, Nitrobacteraceae

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