New multiple-deletion method for the Corynebacterium glutamicum genome, using a mutant lox sequence
Article Abstract:
A new markerless-deletion method based on a Cre/mutant lox system combining a right-element (RE) mutant lox site with a left-element (LE) mutant lax site is used for large-scale genome rearrangements in Corynebacterium glutamicum. The Cre/loxP system using a pair of mutant lox sites provides a new, efficient genome rearrangement method for C. glutamicum and it also facilitates the understanding of genome functions of microorganisms.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Isolation and characterization of a native composite transposon, Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum chromosomal DNA
Article Abstract:
The intrinsic characteristics of a native 20.3-kb composite transposon, Tn14751, which carries approximately 17.4kb of Corynebacterium glutamicum chromosomal DNA is reported. It is concluded that a derivative of this element carrying a kanamycin resistance cassette, minicomposite Tn14751, transposed into the genome of C. glutamicum at an efficiency of 1.8 x 10(super 2) transformants per ug of DNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Large-scale engineering of the Corynebacterium glutamicum genome
Article Abstract:
The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. A precise genome excision method that successfully deleted 11 distinct genomic regions identified by comparative analysis of Corynebacterium glutamicum genomes was developed to construct an improved Corynebacterium glutamicum genome.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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