Nonspecific reactions of a commercial enzyme-linked immunosorbent assay kit (TECRA) for detection of staphylococcal enterotoxins in foods
Article Abstract:
The sensitivity and specificity of a visual immunoassay kit (TECRA) to detect contamination of food with staphylococcal enterotoxins A to E (SEA to SEE) was evaluated. Sensitivity for detection of SEA, SEB and SEC in ham, cheese and mushrooms was found to be comparable to those of kits based on enzyme immunoassay and reversed passive latex agglutination. However, TECRA showed nonspecific reactions to food contaminated with bacteria other than Staphylococcus aureus. The false positive results could be reduced by heating the samples in boiling water for 2 minutes or by using a cleanup procedure with copper chelate Sepharose chromatography prior to using the TECRA kit.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Simple solutions to false-positive staphylococcal enterotoxin assays with seafood tested with an enzyme-linked immunosorbent assay kit (TECRA)
Article Abstract:
The TECRA kit is an enzyme-linked immunosorbent assay system which uses a polyvalent capture antibody against stapylococcal enterotoxin (SE) types A to E. The specificity of the TECRA kit in evaluating SEs in foods was evaluated. The results showed that the TECRA kit is convenient, economical, rapid and sensitive. However, a significant number of false-positive result were obtained when assaying certain seafood extracts. In these cases, treatment with rabbit or calf normal serum, mild heat or copper-chelate-Sepharose gel chromatography is required.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Effect of urea treatment on recovery of staphylococcal enterotoxin A from heat-processed foods
Article Abstract:
Urea treatment fails to recover the heat-inactivated antigenic components of staphylococcal enterotoxin A (SEA) from heat-processed mushrooms and meat products. Food samples treated with urea show lower rates of recovery of toxins than those without urea treatment. The heat resistance of the native SEA serological components is three times the heat resistance of the purified toxin. However, heating to a F(sub o) of 4 to 18 or autoclaving at 121.1 degree celsius breaks down about 92 to 94% of the antigenicity of the toxin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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