Phosphorylation of residue 131 of HIV-1 matrix is not required for macrophage infection
Article Abstract:
Philippe Gallay et al. have proposed that phosphorylation of 1% of the matrix domain (MA) of HIV-1 Gag on a C-terminal Tyr was needed to reverse the membrane binding characteristics of MA and promote an association between MA and the integrase protein, thereby enabling MA to help in the translocation of the HIV-1 preintegration complex to the nucleus. However, experiments designed to duplicate the said findings revealed that the 131YF mutation had no discernible effect in cells from various macrophage donors, in two different primary macrophage purification/culture systems, in two different macrophage-tropic molecular clones and in the presence or absence of a functional vpr gene.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg
Article Abstract:
Bcg is a dominant gene on mouse chromosome 1 that affects the capacity of macrophages to destroy ingested intracellular parasites early during infection. A 400kb bacteriophage and cosmid contig with the gemnomic interval containing Bcg was assembled. Six novel genes in this contig were identified by exon amplification, one of which, designated Nramp, was expressed exclusively in macrophage populations from reticuloendothelial organs and in the macrophage line J774A. The findings suggest that Nramp and Bcg are the same genes, and that this putative transporter plays a key role in general macrophage response to infection.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Hijacking the cell: parasites in the driver's seat
Article Abstract:
Research shows that intracellular parasites have the ability to manipulate the structure and pathways of the host cell so that they can create a better environment for themselves. Among the strategies they use are reconfiguring host compartments and inhibiting or activating host cell signaling pathways for invasion or survival. Parasite genome projects are already being initiated to identify parasite molecules that mediate the events triggered by parasites in the host cell.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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