Plasmids responsible for horizontal transfer of naphthalene catabolism genes between bacteria at a coal tar-contaminated site are homologous to pDTG1 from Pseudomonas putida NCIB 9816-4
Article Abstract:
The naphthalene-catabolic plasmids of phylogenetically diverse bacteria were compared with that of Pseudomonas putida to determine the mechanism of horizontal transfer of the nahAc gene in situ. Findings showed that the plasmids were self-transmissible. Plasmids from 12 site-derived isolates were closely related to each other and to the naphthalene-catabolic plasmid of P. putida. The similarity among all site-derived plasmids and pDTG1 was confirmed by using the entire pDTG1 plasmid as a probe in Southern hybridizations.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Natural horizontal transfer of a naphthalene dioxygenase gene between bacteria native to a coal tar-contaminated field site
Article Abstract:
The sequences of the 16S rRNA and naphthalene dioxygenase iron-sulfur protein (nahAc) genes of nine naphthalene-degrading bacteria isolated from a coal tar waste-contaminated site, including a naphthalene-degrading bacterium from a contaminated site in Washington state and two archetypal naphthalene-degrading strains were compared. Seven strains from the study site had a single nahAc allele, while the 16S rRNA gene sequences of the strains varied by as much as 7.9%.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples
Article Abstract:
Research conducted on the method to obtain DNA from soil that contains organic matter is analyzed. Different purification and extraction procedures are examined.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: The effects of nutrient limitation on styrene metabolism in Pseudomonas putida CA-3. Effect of growth rate, nutrient limitation and succinate on expression of TOL pathway enzymes in response to m-xylene in chemostat cultures of Pseudomonas putida (pWWO)
- Abstracts: A Bacillus cereus member of the SNF2 family. Genome organization is not conserved between Bacillus cereus and Bacillus subtilis
- Abstracts: Nondisruptive detection of activity of catabolic promoters of Pseudomonas putida with an antigenic surface reporter system
- Abstracts: Cell-density-dependent sensitivity of mer-lux bioassay. Conjugal gene transfer to aquatic bacteria detected by the generation of a new phenotype
- Abstracts: One group of genetically similar Listeria monocytogenes strains frequently dominates and persists in several fish slaughter- and smoke-houses