Production of a non-toxic site-directed mutant of Clostridium perfringens e-toxin which induces protective immunity in mice
Article Abstract:
A group of 10 site-directed mutants of Clostridium perfringens e-toxin was produced. All of the mutated proteins expressed in Escherichia coli were identified in immunoblots by a neutralizing mAb raised against wild-type native e-toxin. One mutation caused a loss of activity. This non-toxic protein was obtained by substituting a proline for the histidine at residue 106 of the toxin. Immunization of mice with the non-toxic mutated e-toxin caused a specific antibody response and immunized mice were protected against 1000 LD50 doses of wild-type recombinant e-toxin.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Molecular variation between the alpha-toxins from the type strain (NCTC 8237) and clinical isolates of Clostridium perfringens associated with disease in man and animals
Article Abstract:
The properties of the alpha-toxins produced by isolates of Clostridium perfringens from the intestine or from soft tissues of man and cattle are compared with those of the alpha-toxin from the type strain NCTC 8237. The alpha toxins are shown to differ in their reactions to a neutralizing monoclonal antibody, although the difference had no effect on the enzymic or toxic properties of the protein. A genetically engineered vaccine derived from the alpha-toxin of the strain NCTC 8237 is also shown to provide protection against alpha-toxins from other strains.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Small cytoplasmic RNA (scRNA) gene from Clostridium perfringens can replace the gene for the Bacillus subtilis scRNA in both growth and sporulation
Article Abstract:
Expression of the Clostridium perfringens small cytoplasmic RNA (scRNA) gene induces normal vegetative growth and the formation of heat resistance spores in Bacillus subtilis mutants lacking scRNA. The Escherichia coli 4.5S rRNA causes no sporulation in the B. subtilis mutants. The difference is due to the presence of signal-recognition-particle-like RNA domains I and II in the C. perfringens scRNA gene. Nucleotide sequence and the secondary structure of the C. perfringens and B. subtilis scRNA are similar.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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