Purification and characterization of two serine carboxypeptidases from Aspergillus niger and their use in C-terminal sequencing of proteins and peptide synthesis
Article Abstract:
Two monomeric serine carboxypeptidases (CPD-I and CPD-II) from Aspergillus niger were isolated and characterized. The N-terminal sequence of CPD-I was homologous to that of malt carboxypeptidase II. CPD-I and CPD-II were both acidic glycoproteins, and CPD-II contained a free sulfhydryl group. Both enzymes were specific for esters with Arg, Lys and Phe in the P1 position and catalyzed aminolysis reactions with peptide esters as substrates. Thus, CPD-I and CPD-II could be used for peptide synthesis as well as for protein C-terminal sequencing.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Shifting the pH profile of Aspergillus niger Phy A phytase to match the stomach pH enhances its effectiveness as an animal feed additive
Article Abstract:
Environmental pollution by phosphorus from animal waste is a major problem in agriculture because simple-stomached animals such as swine, poultry and fish cannot digest phosphorus (as phytate) present in plant feeds. To alleviate this problem, a phytase from Aspergillus niger Phy A is widely used as a feed additive to hydrolyze phytate-phosphorus and in conclusion it is feasible to improve the function of Phy A phytase under stomach pH conditions by rational protein engineering.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Transcriptional activation of the Aspergillus nidulans gpdA promoter by osmotic signals
Article Abstract:
An Aspergillus nidulans strain adapted to high sodium chloride concentration contains a glyceraldehyde-3-phosphate dehydrogenase gene (gdpA) that can be induced with gradual adaptation to increasing osmotic concentration. This gene together with the pyr4 gene of Neurospora crassa were used to transform A. nidulans pyrG auxotroph. Determination of marker enzyme levels confirmed the initial findings of osmotic activation of the gdpA promoter.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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