Quantitative spectrophotometric assay for staphylococcal lipase
Article Abstract:
Quantitative assays for lipase activity are generally complicated procedures requiring the use of specialized instruments. A simple, less expensive procedure for the quantitative determination of lipase activity in culture supernatants of Staphylococcus aureus was developed. The procedure exploits the ability of lipase to induce clearing of tributyrin emulsions. Tributyrin clearance is measured in plate assays by spectorphotometric analysis. Tests showed that the procedure is sensitive to 1.0 micrograms of lipase per milliliter, and under the prescribed conditions, is specific for lipase detection.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Purification and partial characterization of a novel antibacterial agent (Bac1829) produced by Staphylococcus aureus KSI1829
Article Abstract:
Staphylococcus aureus KSI1829 produces an antibacterial agent, Bac1829, that is a bacteriocin-like heat stable substance showing activity against different genera of microorganisms. The digestive action of proteases kills this peptide. The stability of Bac1829 is similar to that of other gram-positive bacteriocins. Bac1829 is hydrophobic due to the presence of a large number of hydrophobic amino acids. Purified Bac1829 destroys exponentially growing Corynebacterium renale by a bactericidal mode of action.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Purification and characterization of staphylococcin BacR1, a broad-spectrum bacteriocin
Article Abstract:
The broad-spectrum bacteriocin, staphylococcin BacR1 is purified and characterized by molecular weight determination, amino acid composition and mode of action. This bacteriocin appears to interact with a specific cellular receptor. Staphylococcin BacR1 was found to be bactericidal against many microorganisms. The therapeutic implications of its bactericidal activity are elucidated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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- Abstracts: Characterization of thermophilic consortia from two souring oil reservoirs. Direct cloning from enrichment cultures, a reliable strategy fo isolation of complete operons and genes from microbial consortia
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- Abstracts: Linkage analysis of potyvirus resistance alleles in cucumber. Heart leaf, a recessive leaf shape marker in cucumber: linkage with disease resistance and other traits
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