RNA ligase structures reveal the basis for RNA specificity and conformational changes that drive ligation forward

Article Abstract:

The crystal structures for full-length T4 RNA ligase 2 (Rnl2), alone and bound to duplex substrates, that capture the enzyme at multiple stages along the reaction pathway were determined to clarify outstanding issues concerning the C domain structure, conformational transitions accompanying catalysis, and discrimination of RNA versus DNA damage. The structures illuminate the stereochemistry of nucleotidyl transfer and reveal how remodeling of active-site contacts and conformational changes propel the ligation reaction forward.

RNA, Structure, Nucleotidases

---

The FK506 protein Fp3 counteracts protein phosphatase 1 to maintain meiotic recombination checkpoint activity

Article Abstract:

The FK506 binding protein Fpr3 prevents premature adaptation to damage and thus serves to maintain recombination checkpoint activity. The findings define a branch of the recombination checkpoint involved in the adaptation to persistent chromosomal damage and a critical function for FK506 binding pdroteins during meiosis.

Protein binding, Meiosis

---

Homologous recombination generates T-loop-sized deletions at human telomeres

Article Abstract:

A mutant allele of TRF2, TRF2(super deltaB) is described that suppresses nonhomologous end joining (NHEJ) but induced catastrophic deletions of telomeric DNA. The deletion events occurred rapidly generating shortened telomeres that were accompanied by a DNA damage response and induction of senescence.

Allelomorphism, Alleles, Telomerase

Similar abstracts:

• Abstracts: The robustness of the transcriptional response to alterations in morphogenetic gradients
• Abstracts: Tumor suppressor p53 is a direct transcriptional activator of the human bax gene
• Abstracts: Nef-mediated suppression of T cell activation was lost in a lentiviral lineage that gave rise to HIV-1. Homeostatic expansion of T cells during immune insufficiency generates autoimmunity
• Abstracts: The adipocyte: storage depot or node on the energy information superhighway?
• Abstracts: Male biased sex ratio in the Mediterranean fruit fly Ceratitis capitata, an example of Y-chromosome meiotic drive

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.