Reconstitution of glycophosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco
Article Abstract:
A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) is mapped in order to identify potential split sites by using a transposon-based linker-scanning method. Intein-mediated protein complementation is used for reconstituting glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH
Article Abstract:
RdpA and SdpA were expressed and purified from Sphingomonas herbicidovorans MH as [His.sub.6]-tagged fusion proteins and the kinectic behavior of the enzymes with various substrates and cosubstrates were characterized. It was verified that RdpA and SdpA are two enantioselective alpha-ketoglutarate-dependent dioxygenases belonging to subgroup II by the measurement of enzyme activities with a novel coupled enzyme assay.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Article Abstract:
A Pseudomonas putida strain, 4G-1 was isolated from a soil heavily contaminated by glyphosate in China. The results show that 4G-1 exhibits high tolerance to glyphosate which indicates a protein with a high potential for structural and functional studies of AroA in general and its potential usage for the generation of transgenic crops resistant to the herbicide.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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