Reductive dehalogenase gene expression as a biomarker for physiological activity of Dehaloccoides spp.

Article Abstract:

The transcriptional expression of the reductive dehalogenase (RDase)-encoding tceA and vcrA genes, their applicability as potential biological markers of Dehalococcoides activity is evaluated. When Dehalococcoides ethenogenes 195 is provided with trichloroethene as the electron acceptor, the expression of the tceA gene increases by 90-fold relative to that in cells starved of chlorinated ethenes, demonstrating that tceA gene expression is indicative of the active physiological state of this strain.

Industrial Organic Chemicals, Trichloroethane, Dechlorination, Bacterial genetics, Trichloroethane (1,1,1-trichloroethane), Biological markers

---

Discrimination of multiple Dehalococcoides Strains in trichloroethene enrichment by quantification of their reductive dehalogenase genes

Article Abstract:

The quantitative PCR to 16S rRNA and reductase gene sequences is performed on trichloroethene (TCE) enrichment extracted from the soil taken from the Alameda Naval Air Station (ANAS) to classify and track Dehalococcoides strains by a four-gene plasmid standard. It was observed that the ANAS culture had a single Dehalococcoides strain with two 16S rRNA gene sequence, matching that of D.ethenogenes 195.

California, Industrial organic chemicals, not elsewhere classified, Ethyl Chloride, Analysis, Environmental aspects, Genetic aspects, Bioremediation, Polymerase chain reaction, Marine bacteria, Genetic research

---

Transcriptional expression of the tceA gene in a Dehalococcoides-containing microbial enrichment

Article Abstract:

Dynamic changes in the transcriptional expression of the tceA gene are characterized in a Dehalococcoides-containing microbial enrichment culture. Results are used to examine whether the expression of tceA is regulated at the transcription level or primarily affected by changes in the metabolic state of the tceA-containing organism(s).

Gene expression, Genetic transcription, Transcription (Genetics), Microbial genetics

Similar abstracts:

• Abstracts: Indications for acquisition of reductive dehalogenase genes through horizontal gene transfer by Dehalococcoides ethenogenes strain 195
• Abstracts: Spectrum of the reductive dehalogenation activity of Desulfitobacterium frappieri PCP-1. Purification, cloning, and sequencing of a 3,5-dichlorophenol reductive dehalogenase from Desulfitobacterium frappieri PCP-1
• Abstracts: Suicide polymerase endonuclease restriction, a novel technique for enhancing PCR amplification of minor DNA templates
• Abstracts: Amplified intergenic locus polymorphism as a basis for bacterial typing of Listeria spp. and Escherichia coli
• Abstracts: An endocytic pathway essential for renal uptake and activation of the steroid 25-(OH) vitamin D3. Response: Cellular uptake of sex steroid hormones

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.