Regulation of the phosphate stress response in Rhizobium meliloti by PhoB
Article Abstract:
A research was conducted to study the responses of Rhizobium meliloti to phosphate stress and to determine the phosphate level at which R. meliloti induces its P stress response. Transposon Tn5 was conjugated from Escherichia coli S17-1 into R. meliloti via the suicide vector pSUP2021. Alfalfa seedlings in sterile growth box units were utilized to determine the symbiosis phenotype of the phoB mutant. Results indicated that the loss of a functional PhoB did not disturb the symbiotic function of R. meliloti regardless of the available phosphate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
New recombination methods for sinorhizobium meliloti genetics
Article Abstract:
The availability of bacterial genome sequences has created a need for improved method for sequence-based funtionalized analysis to facilitate moving from annotated DNA sequence to genetic for analyzing the roles that postulated genes play in bacterial phenotypes. A powerful cloning method that uses lambda integrase recombination to clone and manipulate DNA sequences is adapted for use with the gram-negative alpha-proteobacterium sinorhizobium meliloti in two ways.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Development of a functional genomics platform for Sinorhizobium meliloti: Construction of an ORFeome
Article Abstract:
A strategy for genetic analysis of Sinorhizobium meliloti on a genomic scale is presented and the successful implementation of the first step of this strategy is reported. The strategy could be generalized to many other genome projects, and the Sinorhizobium meliloti clones should be useful for investigators wanting an accessible source of cloned genes encoding specific enzymes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Comparison of the bile salts and sodium dodecyl sulfate stress responses in Enterococcus faecalis. Changes in protein synthesis and morphology during acid adaptation of Propionibacterium freudenreichii
- Abstracts: Distinct roles of c-Abl and Atm in oxidative stress response are mediated by protein kinase C delta. VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases
- Abstracts: Quantification of tetracycline resistance genes in feedlot lagoons by real-time PCR. Diverse tetracycline resistance genotypes of megasphaera elsdenii strains selectively cultured from swine feces
- Abstracts: Spatial distribution and stability of the eight microbial species of the altered schaedler flora in the mouse gastrointestinal tract
- Abstracts: Double-layer plague assay for quantification of enteroviruses. Enteroviruses and bacteriophages in bathing waters