Simulation of effects of dominance on estimates of population genetic diversity and differentiation

Article Abstract:

The emergence of PCR-based molecular markers has resulted in a rapid expansion in studies to describe the rates and distribution of genetic variation amongst populations at the DNA level. Randomly amplified polymorphic DNA (RAPD) markers have been useful for population studies, but the rates of genetic variation, differentiation and fine-scale genetic structures often differ. A dominance simulation program, DOM-SIM was developed to asses whether such differences are biological or due to the dominance and biallelism of RAPD and AFLP markers. Dominant biallelic markers, such as RAPDs are likely to strongly underestimate population diversity, but give a reasonable estimate of population differentiation.

Measurement, Biological diversity, Biodiversity, Genetic polymorphisms

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RAPD genome maps of Douglas-fir

Article Abstract:

Douglas-fir has an extra chromosome and therefore a haploid number of 13, according to research designed to assess the genetic length of the Douglas-fir genome and to produce a set of markers to use in mapping quantitative trait loci in linked research into adaptive trait variation between the coastal and interior varieties. Five of the 13 chromosomes have medial centromeres, while six have subterminal centromeres. Two of the smaller chromosomes have terminal centromeres. Many markers will be needed for comprehensive quantitative trait loci and marker-aided selection projects.

Genetic aspects, Douglas fir

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RAPD variation in Norway Spruce seedlot: Consequences of Somatic embryogenesis

Article Abstract:

Random amplified polymorphic DNAs (RAPDs) were used to determine if plant production by somatic embryogenesis eroded the genetic diversity of a provenance. Seedlings from a natural Norway spruce population were divided into two subpopulations, embryogenic and nonembryogenic. It is concluded that the embryogenesis process was not significant in the embryogenic subpopulation, and that erosion of genetic diversity is mostly determined by bottlenecks.

Research, Spruces, Somatic hybrids, Hybrid cells, Norway spruce

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