Heteroduplex panel analysis, a novel method for genetic identification of Aspergillus Section Flavi strains
Article Abstract:
Research has been conducted on the Aspergillus Section Flavi strains. The identification of the Aspergillus Section Flavi species and the genetic diversity within the species have been described.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Sequence variability in homologs of the aflatoxin pathway gene aflR distinguishes species in Aspergillus section flavi
Article Abstract:
PCR amplification and sequencing confirm the presence of the homologs of the DNA-binding domain of the Aspergillus parasiticus aflR gene in Aspergillus section Flavi and various other isolates. A comparative study of the amino acid sequences reveals the presence of a zinc finger domain in various isolates examined. Different finger prints of various species are explained by the 5'-untranslated and zinc finger region having different base substitutions and specific base deletion.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus Section Flavi
Article Abstract:
The polymerase chain reaction-single strand conformation polymorphism analysis is an inexpensive method that reliably distinguishes between species of Aspergillus Section Flavi. The method classifies the strains tested into four groups, regardless of origin - A. flavus/A. oryzae, A. parasiticus/A. sojae, A. tamarii, and A. nomius. The analysis of the internal transcribed spacer regions by this method is consistent with the results of restriction fragment length polymorphism analysis with the polymerase chain reaction products. However, A. nomius is not differentiated from A. parasiticus/A. sojae.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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