Stress tolerance in doughs of Saccharomyces cerevisae trehalase mutants derived from commercial baker's yeast
Article Abstract:
A study has revealed that trehalose accumulation may be the critical determinant in stress tolerance in Saccharomyces cerevisae. An experiment involving trehalose accumulation was conducted. This was done through the use of the gene disruption method on diploid homozygous neutral trehalon mutants found in commercial yeast strains. The process was meant to regulate trehalose cellular levels through the avoidance of hydrolyzing enzymes. The trehalose mutations were able to suppress intracellular trehalose degradation and significantly improve freeze tolerance during the fermentation process.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Lysine-overproducing mutants of Saccharomyces cerevisiae baker's yeast isolated in continuous culture
Article Abstract:
A research was conducted to study the selection of amino acid overproduction mutants of Saccharomyces cerevisiae baker's yeast carried out in continuous culture with increasing concentrations of a toxic amino acid analog. Tubes were utilized to inoculate yeast cells while cells were sporulated and determined by using a CHEF-DRII gel electrophoresis apparatus. Results showed that the isolated mutants appeared to have lost the repression effect of lysine on the gene which encodes the key enzyme of the biosynthetic pathway.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Disruption of the yeast ATH1 gene confers better survival after dehydration, freezing, and ethanol shock: potential commercial applications
Article Abstract:
A strain of the yeast Saccharomyces cerevisiae is constructed by abolishing the activity of the trehalose-hydrolyzing enzyme acid trehalase (ATH). The loss of ATH activity is achieved by disrupting the ATH1 gene to obtain a strain with a higher cell density than the isogenic wild-type strain. This strain exhibits excellent tolerance to dehydration, freezing and toxic levels of ethanol.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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