The Saccharomyces cerevisiae Ku autoantigen homologue affects radiosensitivity only in the absence of homologous recombination

Article Abstract:

The recombination of DNA sequences in Saccharomyces cerevisiae was analyzed to identify possible differences between the DNA repair processes of higher and lower mammalian cells. Specifically, the phenotype conferred by a deletion of HDF1, which is a homologue of the 70-kD subunit of the DNA-end binding Ku complex of DNA-PK was studied. The phenotype exhibited non-sensitivity to ionizing radiation or methyl methanesulfonate (MMS). However, if homologous recombination was disabled, the phenotype became sensitivity to both radiation and MMS. The results suggest that lower cells such as yeast are inclined to use homologous recombination of double-strand break repair.

Physiological aspects, DNA repair, Saccharomyces

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Tn7 transposition creates a hotspot for homologous recombination at transposon donor site

Article Abstract:

Tn7 transposition at high frequency in Escherichia coli had been found to have a 10-fold increase in homologous recombination. The increase were noted between two lacZ heteroalleles when one of the lacZ copies have a transposing Tn7. The conversion events of recombination occur preferentially at Tn7 which supports the model that Tn7 transposition uses a 'cut and paste mechanism' wherein repair of Tn7 induced double-strand breaks is by homologous recombination.

Evaluation, Translocation (Genetics), Transposons

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Dependence of frequency of homologous recombination on the homology length

Article Abstract:

Linear dependence is observed in the homology length (N bp) between DNAs for the case of homologous recombination frequency. However, steeper dependence is observed for mammalian gene targeting systems. A model has been developed that assumes a random walk for the reaction factor branch point and to integrate both dependence types. A proportional relationship is observed for N based on functional size.

Homology (Biology), Genetics

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