Structural changes and interactions involved in the Ca(super.2+)-triggered stabilization of the cell-bound cell envelope proteinase in Lactococcus lactis subsp. cremoris SK11
Article Abstract:
Structural changes and interactions that are part of the Ca(super.2+)-triggered stabilization of the cell envelope proteinase (CEP) that is cell-bound in the cheese starter Lactococcus lactis subsp. cremoris SK11 are discussed. It appears that in the Ca-free CEP the absence of local calcium-mediated backbone rigidity and neutralization of negative electrostatic potentials in the weak Ca-binding sites increase the relative effectiveness of electrostatic repulsion forces on the protein enough to cause the instability that is seen. The lack of significant hydrophobic stabilization also has a role. The proteinase is the only extracellular cell surface proteinase of the organism and is required for normal growth in milk.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Purification and characterization of cystathionine beta-lyase from Lactococcus lactis subsp. cremoris B78 and its possible role in flavor development in cheese
Article Abstract:
An enzyme isolated from Lactococcus lactis subspecies cremoris B78 resembles Cystathionine beta-lyase and breaks down sulfur-containing amino acids. This enzyme is probably involved in inducing flavor in cheese. The enzyme of 130 to 165 kilo dalton comprises four identical subunits. It catalyzes alpha,gamma-elimination reactions producing methanethiol from methionine which gives origin to the flavor compounds in cheese.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Mitochondrial function in cell wall blycoprotein synthesis in Saccharomyces cerevisiae NCYC 625 (wild type) and (rho(super.0)) mutants
Article Abstract:
Mitochondrial function in cell wall glycoprotein synthesis in Saccharomyces cerevisiae NCYC 625 (wild type) and (rho(super.0)) mutants has been studied. Phosphopeptidomannans (PPMs) were studied. The aerobic wild-type cultures were very flocculent. All others were weaky flocculent. Ligands implicated in flocculation of mutants or antimycin-treated cells were not aggregated as much by concanavalin A as those of the wild type. Alterations of PPM structures may be responsible for reduced flocculation of cells grown in certain conditions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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- Abstracts: Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363. Six putative two-component regulatory systems isolated from Lactococcus lactis subsp. cremoris MG1363
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