Surface display of the cholera toxin B subunit on Staphylococcus xylosus and Staphylococcus carnosus
Article Abstract:
The surface display of the cholera toxin B (CTB) subunit on Staphylococcus xylosus and Staphylococcus carnosus from the gram negative bacterium Vibrio cholerae was investigated. The study made use of previously reported surface display systems and chimeric receptors containing the CTB subunit which are connected to the cell surface areas from Staphylococcus aurea protein A. Results reveal the occurrence of surface expression of proteins and its need to form subunits to become functional and become means for targeting bacteria for subunit vaccine purposes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Staphylococcal surface display of immunoglobulin A (IgA)- and IgE-specific in vitro-selected binding proteins (affibodies) based on Staphylococcus aureus protein A
Article Abstract:
Research was conducted to determine whether the immunoglobulin A (IgA)- and IgE-reactive affibodies could be expressed in an active form as parts of chimeric surface proteins on Staphylococcus carnosus. The recombinant surface proteins were found to be expressed as full-length proteins, localized and properly exposed at the cell surface of S carnosus. The chimeric proteins were also found to be functional since recombinant S carnosus cells were observed to have gained IgA and IgE binding capacity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Generation of metal-binding staphylococci through surface disp-lay of combinatorially engineered celluloase-binding domains
Article Abstract:
Researchers created a version of Staphylococcus carnosus that binds nickel by introducing a genetically engineered fungal cellulose-binding domain (CBD) from Trichoderma reesei cellulase. The CBD do longer bound cellulose but was able to bind metals. Metal-binding bacteria could be used in biofilters or biosensors.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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