The Drosophila clock gene double-time encodes a protein closely related to human casein kinase I epsilon
Article Abstract:
The Drosophila clock gene double-time is closely related to human casein kinase epsilon. The gene's S and L mutants, which alter the period length of Drosophila circadian rhythms, produce single amino acid changes in conserved regions of the predicted kinase. The P mutants, which eliminate period and timeless expression and overproduce hypophosphorylated PER proteins, abolish most double-time expression. The double-time protein is capable of binding to period proteins in vitro. These suggest that a physical association of period and double-time proteins regulate period phosphorylation in vivo.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
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Double-time is a novel Drosophila clock gene that regulates period protein accumulation
Article Abstract:
The short and long-period alleles of the Drosophila clock gene, doubletime, exhibited both behavioral rhythmicity and molecular oscillations. A third allele has been found to cause pupal lethality and eliminates circadian cycling of period and timeless gene products in larvae. These mutants' period proteins constitutively accumulate, remain hypophosphorylated and no longer depend on timeless proteins for their accumulation. These suggest that the normal function of doubletime protein is to reduce the stability and the level of accumulation of monomeric period proteins.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
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