ThrH, a homoserine kinase isozyme with in vivo phosphoserine phosphatase activity in Pseudomonas aeruginosa
Article Abstract:
A novel gene called thrH encodes a protein with an in vivo homoserine-kinase-like activity in Pseudomonas aeruginosa when expressed in Escherichia coli thrB mutant strains. Threonine auxotrophy is observed only in Pseudomonas aeruginosa when both thrB and thrH are simultaneously activated. Overexpression of the new gene also allows complementation of serine auxotrophy of Escherichia coli and Pseudomonas aeruginosa serB strains. In addition, sequence homology is observed between thrH and the serB proteins from different organisms.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Involvement of the N- and C-terminal domains of Mycobacterium tuberculosis KatG in the protection of mutant Escherichia coli against DNA-damaging agents
Article Abstract:
Research was conducted to examine the involvement of the Mycobacterium tuberculosis katG gene in the protection of DNA-repair-deficient Escherichia coli mutants against DNA-damaging agents. Since there are no defined M. tuberculosis DNA-repair mutants, heterologous hosts were used in the study. Results based on the preliminary screening of an M. tuberculosis genomic DNA library on EMS indicate that the katG gene may be involved in DNA repair.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Aluminum elicits exocellular phosphatidylethanolamine production in Pseudomonas fluorescens. Pseudomonas fluorescens adhesion and transport through porous media are affected by lipopolysaccharide composition
- Abstracts: The use of fluorogenic substrates to measure fungal presence and activity in soil. Identification and retting efficiencies of fungi isolated from dew-retted flax in the United States and Europe
- Abstracts: LAG2, a gene that determines yeast longevity. Pheromone response in trehalose-6-phosphate synthase yeast mutants
- Abstracts: Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form
- Abstracts: O-antigenic determinants in Salmonella species of serogroup C1 are expressed in distinct immunochemical populations of chains