Aluminum elicits exocellular phosphatidylethanolamine production in Pseudomonas fluorescens

Article Abstract:

Phosphatidylethanolamine (PE) participates in the detoxification of aluminum during the stationary phase of growth in Pseudomonas fluorescens. The isolation of aluminum associated with PE from the spent fluid indicates the consumption of citrate and the removal of the metal from the early stages of growth. Precipitation of aluminum and its organic complex with PE occurs when the concentration of these species reaches a critical point. The fact that the aluminum containing cells do not efflux the metal in the absence of citrate suggests that the process is energy dependent.

Usage, Aluminum, Aluminum (Metal), Metabolic conjugation

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Pseudomonas fluorescens adhesion and transport through porous media are affected by lipopolysaccharide composition

Article Abstract:

The composition of the surface lipopolysaccharide (LPS) influences the adhesiveness and movement of Pseudomonas fluorescens through a porous media. Mutants of P. fluorescens with altered surface proteins have greater adhesion to hydrophobic surfaces and lesser adhesion to water-wettable surfaces. Polyacrylamide gel electrophoresis suggests that the LPS of the mutants lacks the O antigen. This exposes the lipid moiety of the LPS which increases the adhesiveness and transport through porous media.

Endotoxins

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Heterobinary adhesins based on the Escherichia coli FimH fimbrial protein

Article Abstract:

A heterobifunctional adhesin was engineered based on the Escherichia coli FimH adhesin. The primary binding domain of FimH recognizes D-mannoside residues. The addition of histidine clusters created a secondary binding site which recognizes metals. Transformed bacteria expressing the recombinant adhesin are able to bind saccharides and metal. Further, the binding of the two domains are independently regulated.

Physiological aspects, Escherichia coli, Cell adhesion molecules

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