Two distinct pathways for trehalose assimilation in the yeast saccharomyces cerevisiae

Article Abstract:

The assay of invertase on whole cells with NaF is used to show that more than 90 percent of the activity of Ath1p is extracellular, splittings of the disaccharide into glucose. It was also found that Agt1p-mediated trehalose transport and the hydrolysis of the disaccharide by the cytosolic neutral trehalase Nth1p are coupled and represent a second, independent pathway, although there are several constraints on this alternative route.

Food preparations, not elsewhere classified, All Other Miscellaneous Food Manufacturing, Brewers' Yeast, Microbiology, Brewer's yeast, Saccharomyces cerevisiae

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Saccharomyces cerevisiae JEN1 promoter activity is inversely related to concentration of repressing sugar

Article Abstract:

Results show that sugars such as glucose, fructose, and mannose repress JEN1 promoter activity in Saccharomyces cerevisiae, which encodes a lactate-pyruvate transporter. Data indicate that JEN1 activity is linear with glucose concentration and that its promoter activity repression is specific to carbon source whereas heat or cold shock, osmotic stress, DNA damage, or nitrogen starvation do not significantly affect the activity.

Physiological aspects, Genetic regulation, Promoters (Genetics), Metabolic regulation

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Structural characterization of glucooligosaccharide oxidase from Acremonium strictum

Article Abstract:

Glucooligosaccharide oxidase from Acremonium strictum is isolated for potential applications in oligosaccharide acid production and carbohydrate detection. The protein is a unique covalent flavoenzyme and the kinetic measurements have indicated that the enzyme possess an open carbohydrate-binding groove, which is mainly composed of two glucosyl-binding subsites.

Enzymes, Microbiological chemistry, Hydrolases, Chemical properties

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