Use of commercial enzyme immunoassays and immunomagnetic separation system for detecting Escherichia coli 0517 in bovine fecal samples
Article Abstract:
The use of commercial enzyme immunoassays (EIA) and immunomagnetic separation (IMS) systems for detecting Escherichia coli 0157 in bovine fecal samples was studied to evaluate the use of a commercial EIA and a commercial culture confirmation system. The study compared the commercial enzyme immunoassay on enrichment cultures in E. coli to the IMS in enrichment cultures in a modified buffered peptone water in E. coli detection. Tests reveal that EIA and IMS methods were more sensitive that direct culture or enrichment subculture methods for organism detection
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Role of fadR and atoC(Con) mutations in poly(3-hydroxybutyrate-Co-3-hydroxyvalerate) synthesis in recombinant pha+ Escherichia coli
Article Abstract:
Significant levels of 3-hydroxyvalerate(3HV) can be incorporated into the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by using recombinant Escherichia coli fadR atoC(Con) mutants containing the polyhdroxyalkanoate biosynthesis genes from Alcaligenes eutrophus. The fadR mutation enhances the levels of fatty acid enzyme whereas, atoC(Con) mutation increases propionate uptake into the cells. The activity of fadR depends upon fadB+ and fadA+ gene products.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Unidirectional motility of Escherichia coli in restrictive capillaries
Article Abstract:
Escherichia coli K-12 cells may only swim in one direction in a capillary tube with a 6 micrometer diameter. The bacteria in such a tube did not move in a direction parallel to the walls of the tube but were unable to turn around, probably because their flagella were too long and rigid. The bacteria swam at an average speed of 11.4 micrometers per second and rarely turned more than 90 degrees.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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- Abstracts: Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157:H7 in aqueous samples and comparison of the assay with a standard microbiological method
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