Knockdown by RNAi-proceed with caution
Article Abstract:
Studies suggest that the small interfering ribonucleic (RNA) (siRNAs) that are synthesized by phage polymerases but not by chemical methods, activate the interferon system (IFN) and could complicate the interpretation of RNA interference (RNAi) gene knockdown experiments in mammalian cells. The role of the 5'-triphosphate end of the in vitro phage polymerase-transcribed siRNA in inducing IFN is described and its results suggest that 5'-triphosphate end of the RNA transcribed in vitro plays a putative role in determining IFN inducing capacity.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
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All in the RNA family: Small interfering RNA is targeted to a specific cell type in vivo using an RNA aptamer
Article Abstract:
Proof-of-principle studies demonstrate that RNA aptamers, RNA ligands that bind specific receptors, can be covalently coupled with siRNAs to achieve cell-selective silencing in vivo. This 'all RNA' approach combines the power of RNA aptamers for cell targeting with the utility of RNAi for directed gene silencing in a single oligonucleotide.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2006
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