Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins
Article Abstract:
The role of coat proteins in Golgi membrane budding was investigated. The membrane-mediated budding hypothesis states that budding is an intrinsic property of Golgi membranes which can thus proceed without extrinsic coat proteins. On the other hand, the coat-mediated budding hypothesis states that coat proteins are essential for budding to occur. The study involved a cell free system in which coat proteins formed from Golgi cisternae are immunodepleted. The results proved that coat proteins are essential for Golgi membrane budding.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF
Article Abstract:
A new Golgi membrane enzyme that assists in the guanine nucleotide (GDP-GTP) exchange within the adenosine diphosphate-ribosylation factor has been identified through the ability of brefeldin A (BFA), a fungal metabolite, to block the exchange. BFA's inhibitory effect is strong evidence for an enzymatic nucleotide swapping device that is necessary for the development of budding vesicles.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992
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Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein
Article Abstract:
Brefeldin A (BRA), a fungal metabolite, disrupts the Golgi membrane-controlled catalysis by which GTP-binding protein is swapped for adenosine diphosphate-ribosylation factor (ARF). BRA effects this disruption by binding to guanine nucleotide. BRA's inhibitory property suggests the existence of a Golgi-linked binding protein whose chief function is to link ARF with guanine nucleotide.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992
User Contributions:
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