Purification of an MCM-containing complex as a component of the DNA replication licensing system
Article Abstract:
Replication licensing factor (RLF) was purified from Xenopus eggs and its structure and function were characterized. RLF is a eukaryotic cell protein that ensures that DNA replication occurs only once during each cell cycle. It is activated after exit from metaphase, binds and modifies chromatin to authorize the subsequent replication and is inactivated during replication. Since it is not transported into the nucleus, DNA can come into contact with it only during mitosis. Xenopus RLF is comprised of RLF-M and RLF-B, both of which are required for replication licensing. RLF-M is comprised of three polypeptides, one of which is a homologue of the yeast MCM3 protein.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells
Article Abstract:
A Xenopus homologue of the MCM family of yeast replication proteins that have been characterized as the putative replication licensing factor in eukaryotic cells was purified from Xenopus eggs. Antibodies were raised against the Xenopus MCM3 and used in immunodepletion studies in Xenopus sperm nuclei and HeLa nuclei. The results show that Xenopus MCM3 accumulates in cell nuclei before replication but decreases during replication, indicating that MCM3 could be the replication licensing factor in eukaryotic cells.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
A new check on issuing the licence
Article Abstract:
It has been established that the protein known as Cdt1 is needed for origin licensing. Overexpressed Cdt1 cannot induce over-replication by itself, suggesting that it may need to be less tightly repressed later in the cell cycle than is Cdc6/Cdc18.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Purification and unique properties of mammary epithelial stem cells. Nonindependence of mammalian dental characters
- Abstracts: Purification and unique properties of mammary epithelial stem cells. part 2 Generation of a functional mammary gland from a single stem cell
- Abstracts: Holliday junctions cleaved by Rad1? Structure of a multisubunit complex that promotes DNA branch migration. DNA helicases get physical
- Abstracts: Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate. Cryptic sites in vinculin
- Abstracts: Sex-specific selection on time to remate in Drosophila melanogaster. Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products