Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum
Article Abstract:
The construction of a Cre-lox-based system for multiple gene deletions in Lactobacillus plantarum that could be adapted for use on gram-positive bacteria is presented. This multiple gene deletion system for gram positive bacteria allows for effective, standardized construction of double-crossover mutants that could be introduced in a single genetic background by a simple repetitive procedure using mutant lox sites of Cre-mediated selectable-marker removal.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
Identification and genetic characterization of a novel proteinase, PrtR, from the human isolate Lactobacillus rhamnosus BGT10
Article Abstract:
Research describes the cloning, sequencing, and characterization of the prtR gene encoding a novel type of cell envelope proteinase, PrtR, from the human vaginal Lactobacillus rhamnosus BGT10. Results indicate that the prtR expression is Casitone dependent, suggesting that its transcription is enhanced under depleted nitrogen in conformation with the enzyme's catalytic activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
High-level production of the low calorie sugar sorbitol by Lactobacillus plantarum through metabolic engineering
Article Abstract:
The capacity of Lactobacillus plantarum to produce sorbitol from frutose-6-phosphate by reverting the sorbitol catabolic pathway in a mutant strain deficient for both L- and D- lactate dehydrogenase activities is investigated. It was found that the two sorbitol-6-phosphate dehydrogenase genes were constitutively expressed at a high level in the mutant strain.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Corrosion and electrochemical oxidation of a pyrite by Thiobacillus ferrooxidans. Solid-phase products of bacterial oxidation of arsenical pyrite
- Abstracts: PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells. Formation of pseudo-terminal restriction fragments, a PCR-related bias affecting terminal restriction fragment length polymorphism analysis of microbial community structure
- Abstracts: Characterization of genes involved in the metabolism of alpha-galactosides by Lactococcus raffinolactis. Role of galK and galK in galactose metabolism by Streptococcus thermophilus
- Abstracts: PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells. part 2 Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples
- Abstracts: A most-probable-number assay for enumeration of infectious Cryptosporidium parvum oocysts. Use of cell culture to assess Cryptosporidium parvum survival rates in natural groundwaters and surface waters