In response to Freed et al
Article Abstract:
The suggestion that the C-terminal tyrosine phosphorylation of the matrix domain (MA) of HIV-1 Gag shows the karyophilic potential of this protein in HIV-1 preintegration complex was based on a series of experiments which identified the components of this complex and determined their interactions among each other and with the cell nuclear import system. The experiments were reinvestigated and the contention of Eric O. Freed et al. that MA tyrosine phosphorylation does not help in facilitating HIV-1 nuclear import is incorrect. The discrepancies may have been in the application of different multiplicities of infection.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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HIV-1 infection of nondividing cells: C-terminal tyrosine phosphorylation of the viral matrix protein is a key regulator
Article Abstract:
Myristoylation of the N-terminus of HIV matrix (MA) protein, which has two subcellular localization signals, serves as the MA dominant targeting signal in cells producing HIV-1. A subset of MA undergoes phosphorylation in the presence of a viroin-associated cellular protein kinase during the assembly of the virus. Tyrosine phosphorylation of MA indicates the karyophilic characteristics of the MA protein in the preintegration complex of HIV-1, and the contribution of MA protein to the infection of nondividing cells.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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HIV nuclear import is governed by the phosphotyrosine-mediated binding of matrix to the core domain of integrase
Article Abstract:
Tyrosine-phosphorylated viral matrix (MA) proteins are incorporated into the the viral nucleoprotein complexes which are actively transported into the nonmitotic host cells. Phosphorylated MA proteins bind to integrase which allows their incorporation into the nucleoprotein complexes. The nuclear import of HIV proteins is controlled by the karyophilic properties of the MA proteins. The movement of HIV proteins into nonmitotic cells such as macrophages is necessary for viral transmission and development of AIDS.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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