Incorporation of [(super 15)N] ammonia by the cellulolytic ruminal bacteria Fibrobacter succinogenes BL2, Ruminococcus albus SY3, and Ruminococcus flavefaciens 17
Article Abstract:
Some cellulolytic ruminal bacteria incorporate nitrogen from non-ammonia sources depending on the growth medium. Amino acids can also stimulate their growth on certain media. This shows that amino acids are a significant source of nitrogen for these bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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De novo synthesis of amino acids by the ruminal bacteria Prevotella bryantii B(sub 1)4, Selenomonas ruminantium HD4, and Streptococcus bovis ES1
Article Abstract:
Research was conducted to examine the influence of peptides and amino acids on ammonia assimilation and de novo synthesis of amino acids by three predominant noncellulolytic species of ruminal bacteria, namely, Prevotella bryantii, Streptococcus bovis ES1 and Selenomonas ruminantium HD4. The bacteria were grown in media with 15 NH4Cl and additional pancreatic hydrolysates of casein or amino acids. Results suggest a wide variation in de novo synthesis between individual amino acids and individual species.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Influence of 1-((E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl)pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms
Article Abstract:
The consequences of 1-((E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl)pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were investigated in a mixed rumen microbial population obtained from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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