Is epimorphin involved in vesicular transport?
Article Abstract:
Epimorphin cDNA, which has been tagged at the extreme C-terminus with a c-Myc epitope, was expressed in COS cells. A 34-kd protein was identified with anti-Myc antibodies. Moreover, the epitope was accessible on the plasma membrane of intact cells, indicating a membrane orientation similar to that of other members of the syntaxin family. In contrast, untagged epimorphin expressed in COS cells yielded a 34 kd protein that is detectable with anti-syntaxin antibodies. However, no proteins in this expression experiment were bound to the monoclonal antibody MC-1. Two alternative explanations are proposed for these observations.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Reexamination of the properties of epimorphin and its possible roles
Article Abstract:
A fusion protein of the mouse epimorphin cDNA product and beta-galactosidase was prepared, using the pEX1 vector, and immunized rats to generate antibodies. This was done to reexamine whether the cDNA encodes for the 150 kd protein expressed in mesenchymal cells. The results suggest that the protein is the major antigen present in two forms, trypsin-resistant and trypsin-sensitive. The relation of these findings to a function for epimorphin in vesicular transport is discussed.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum
Article Abstract:
Ufe1p, a novel yeast integrated membrane protein, mediates the retrograde traffic of transport vesicles from the Golgi complex to the endoplasmic reticulum (ER). The Ufe1p protein, which has structural and sequence similarity to known t-SNAREs (target membrane proteins), is located in the ER. Mutation of the Ufe1p protein also interferes with ER-Golgi complex forward traffic.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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