The ParB protein encoded by the RP4 par region is a Ca2+-dependent nuclease linearizing circular DNA substrates
Article Abstract:
A study was conducted on the functional characterization of the ParB nuclease encoded by the RP4 par region. The findings indicate that cleavage of circular DNA substrates occurs preferentially at specific sites. Moreover, the analysis of ParB-linearized plasmid DNA shows a specific target for ParB action adjacent to an AT-rich region containing palindromic sequence elements on a pBR322-derived plasmid.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Isolation of Aspergillus niger creA mutants and effects of the mutations on expression of arabinases and L-arabinose catabolic enzymes
Article Abstract:
A study was conducted on four Aspergillus niger creA mutants relieved of carbon repression to determine whether their metabolic systems are controlled by repressor protein CREA. The mutants arabinase and L-arabinose expressions were analyzed using Western blotting. Results have shown that the CREA is involve in the D-glucose repression of arabinases and L-arabinose catabolism.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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